ECM2001 MANUAL PDF

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The Legacy ECM is capable of producing a proprietary AC waveform for benign dielectrophoretic alignment of cells, and a square DC waveform for cell fusion. Microsecond switch-over time manula AC to DC allows for highly efficient fusion. Multi-purpose electro cell manipulation generator capable of producing a proprietary AC waveform for electrophoretic alignment of cells and a square DC waveform for cell fusion.

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After fusion, the AC pulse reapplication ecm2010 compression of the cells following the DC pulse resulting in a higher number of hybrids. Utilizing the Legacy ECM for standard mammalian cell transfections is simple; the AC feature is turned off and the DC mode is set as a square wave electroporation device.

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The LEGACY ECM is a multipurpose electro cell manipulation pulse generator capable of performing both electrofusion for hybridoma production, hybrid cell formation and nuclear transfer applications in addition to electroporation of cells in suspension and in vivo applications.

The 1 MHz AC pulse aligns cells together in real time, saving time seconds vs. The Legacy ECM coupled with the Petri Pulser electrode or the Petri Dish electrode allows researchers to avoid the trypsinization of their cell by electroporating adherent cells directly in the dish they are growing in.

The range of voltages and pulse lengths that can be programmed, coupled with the ability to carry out up to 9 pulses per experiment, make this an extremely versatile system for any lab. Proprietary blend; sterile filtered and made of the highest quality medical-grade chemicals.

Supplier BTX show all products. Blastomeres and oocytes can also be aligned to the correct position for better fusion accuracy during nuclear transfer and embryo manipulation.

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Electrofusion is carried out by applying both the AC and DC pulse waveforms. This system does not require proprietary fusion medium, but can be used with the Cytofusion Medium C for convenience. After fusion, the AC pulse reapplication maintains compression of the cells following the DC pulse for the rounding off process; resulting in a higher number of hybrids.

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Not for use in diagnostic, pre-clinical, or clinical procedures. The Petri Pulser is ideal exm2001 6-well plates and the Petri Dish Electrode is ideal for mm petri dishes.

Manual Ecm – Documents

Electrofusion is achieved by the generation of a proprietary 1 MHz AC waveform for benign dielectrophoretic cell alignment, microsecond switchover from AC to DC creates efficient fusion results. Electroporate adherent cells directly into the dish used for cell growth. For use in hybridoma production applications, the Legacy ECM generator, 3. This versatile platform combines microprocessor-controlled precision with a wide range of possible parameters for a manua range of applications when combined with standard cuvettes, high throughput plates, specialty electrodes and other available accessories.

Cell fusion Nuclear transfer Embryo manipulation Hybridoma production Mammalian cell transfection Plant protoplast fusion Stem cell production.